作者：郝帅nsclc2deoxyglucoselungcancercellline
摘要：Objective To explore the role and mechanism of 2-deoxyglucose ( 2-dg) in reversing osimertinib-acquiredresistance of non-small cell lung cancer ( NSCLC) cellline. Methods The NSCLC line H1975 (purchased fromthe American Type Culture Collection) was conducted byinduction method in vitro to construct the osimertinib-resistanceNSCLC cell line H1975-OR. The osimertinib-resistanceof H1975-OR cell line was examined by 3-(4,5-Dimethylthiazol-2-yl )-2,5-diphenyltetrazolium bromide(MTT) assay,colony-formation assay,Ki67 incorporationassay and the expression of apoptosis-related protein.The glycolysis level was assayed by the lactic acidproduction measured in the culture medium supernatantof H1975 and H1975-OR. The expression of glycolysiskey enzymes (HK2,GLUT1,P-PKM2) and apoptosisrelatedprotein (BIM,Bcl-2) were detected by Westernblot.
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