embryopoisoningviablefluorideexposedlanzhouagentscrownlengthmorphogenetic
摘要：2007141 Toxicity of combination of fluoride and aluminum on rat embryos in vitro. CHEN Li(陈丽), et al. Dept Toxicol, Public Health Coll, Lanzhou Univ, Lanzhou 730000. Chin J Endemiol 2007;26(2):133-138. Objective To explore developmental toxicity and mechanism of combination of fluoride and aluminum to embryos of SD rat. Methods Embryos of SD rat on day 9.5 of gestation were explanted and cultured in a whole-embryo culture system exposed to a series of doses of fluoride and aluminum (F + Al), which were (0 + 0), (5.0 + 0), (5.0 + 1.2), (5.0 + 2.4), (10.0 + 0), (10.0 + 1.2); (10.0 + 2.4), (0 + 1.2) and (0 + 2.4) mg/L. After 48 h of culture, yolk sac diameter, crown-rump length, head length and dry weight of each viable embryo were evaluated by Browns morphological scoring system. Factorial experimental analysis of 3×3 was used to analyze the types of combined action. Results When exposed to both F and Al, yolk sac diameter, crown-rump length and dry weight affected each other (F=29.75,P<0. 01 ), especially with the consistent increase of Al. Every morphogenetic differentiation index has alternate action (P<0.05). Comparison of the data in groups of F + Al of (10.0 + 2.4)mg/L to (10.0 + 0)mg/L showed that Al had antagonism to teratogenic toxicity induced by fluoride. Conclusion Both fluoride and aluminum are developmental toxicant and dysmorphogent to rats. Aluminum partially antagonizes developmental toxicity induced by fluoride on whole embryo culture.
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