作者：翟木绪estradioleffectsof17connectivestainedinteractingalkalinecollagenmaturationstainingmineralization
摘要：Objective To observe the expressions of connective tissue growth factor (CTGF) and polyadenylate-binding protein interacting protein-1 (PAIP-1) mRNA during MG-63 cell proliferation and differentiation, and to investigate the effect of 17β-estradiol (E2) on the expressions of CTGF and PAIP-1 mRNA. Methods The expressions of typeⅠcollagen, alkaline phosphatase (ALP) and osteocalcin mRNA were determined by semiquantitative RT-PCR. Cultured cells were stained with
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