作者：夏运风mesangialexcretiontransfectioncollagenexpressingthymidineeukaryoticcultureddeterminingincorporation
摘要：Objective To investigate the effects and mechanism of Megsin gene transfection on mesangial cell proliferation and typeⅣcollagen excretion. Methods Rat Megsin cDNA eukaryotic expressing vector was constructed and transfected to cultured rat mesangial cells. Cell proliferation was measured by determining [3H] -thymidine (3H-TdR) incorporation. The mRNA expression of
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