作者：FANG; Cong; CHEN; Lan-ying; LUO; Ying-...lungcancerglycolysishypoxiainducible
摘要：OBJECTIVEα-Hederin is an effective component of the traditional Chinese medicine Pulsatilla chinensis,which has been reported to exert many pharmacological activities.However,the effect ofα-hederin on metabolism is still unclear.This study aimed to illuminate the role ofα-hederin in glucose metabolism in lung cancer cells and investigate the molecular mechanism ofα-hederin.METHODS CCK8 and colony formation assays were employed to assess the anti-proliferative effects induced byα-hederin.Glucose uptake,ATP generation,and reduced lactate production were measured using kits,and an A549 tumor xenograft mouse model of lung cancer was used to assess the in vivo antitumor effect ofα-hederin(5,10 mg·kg^-1).Glycolytic-related key enzymes were detected by Western blotting and immunohisto⁃chemical staining.RESULTS Cell proliferation was significantly inhibited byα-hederin in a dose-dependent manner and thatα-hederin inhibited glucose uptake and ATP generation and reduced lactate production.Furthermore,α-hederin remarkably inhibited hexokinase 2(HK2),glucose transporters 1(GLUT1),pyruvate kinase M2(PKM2),lactate dehydro⁃genase A(LDHA),monocarboxylate transporter(MCT4),c-Myc,and hypoxia inducible factor-1α(HIF-1α)protein expres⁃sion.Using inhibitors,we proved thatα-hederin inhibits glycolysis by inhibiting glycolytic regulators.Moreover,a tumor xenograft mouse model of lung cancer further confirmed thatα-hederin inhibits lung cancer growth via inhibiting glycolysis in vivo.CONCLUSIONα-Hederin inhibits the growth of non-small cell lung cancer A549 cells by inhibiting glycolysis.The mechanism of glycolysis inhibition includesα-hederin inhibiting the expression of the glycolytic regulatory factors HIF-1α and c-Myc.
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