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Spotted Sea Bass(Lateolabrax maculatus)cftr,nkcc1a,nkcc1b and nkcc2:Genome-Wide Identification,Characterization and Expression Analysis Under Salinity Stress

作者:ZHANG; Kaiqiang; ZHANG; Xiaoyan; WEN; ...cftrnkccslateolabraxmaculatussalinitygeneexpression

摘要:The Na^+/K^+/2Cl^-cotransporter(NKCC)and the cystic fibrosis transmembrane conductance regulator(CFTR)proteins play crucial roles in the transportation of Na^+and Cl^-.In this study,we identified cftr,nkcc1 a,nkcc1 b and nkcc2 in spotted sea bass(Lateolabrax maculatus)genomic and transcriptomic databases.We also characterized these genes via phylogenetic and structural analyses.The results showed that both cftr and nkcc were highly conservative in L.maculatus.Quantitative real-time polymerase chain reaction(qRT-PCR)analysis in ten tissues showed that cftr,nkcc1 a and nkcc2 highly express in osmoregulatory organs such as gill,kidney and intestine.Furthermore,the expressions of cftr and nkcc1 a in gill as well as nkcc2 in intestine were up-regulated by high salinity,indicating that these genes function potentially in osmoregulation.Our findings provided the insights into the cftr and nkcc functions in euryhaline teleost.

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中国海洋大学学报·自然科学版

《中国海洋大学学报·自然科学版》(CN:37-1414/P)是一本有较高学术价值的大型月刊,自创刊以来,选题新奇而不失报道广度,服务大众而不失理论高度。颇受业界和广大读者的关注和好评。 《中国海洋大学学报·自然科学版》主要刊登理、工、农(水产)、医(药)等涉海相关领域中具有原创性的科技论文。坚持为理论研究、高技术开发、成果转化等专家学者、科技工作者服务,以理论研究和知识创新为主导,兼顾应用于开发等国内、国际的最新研究成果。

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