The Inhibitory Effects of PSS-Loaded Nanoparticles on the Dysfunction of Cardiac Microvascular Endothelia in Rats with Diabetic Cardiomyopathy

作者：AN; Nina; YU; Luyan; HU; Yi; FENG; Wen...nanoparticlesmarinedrugdcmmicrovascularendothelialdysfunction

摘要：Propylene glycol alginate sodium sulfate-loaded nanoparticles(PSS-NP)has been shown potential to prevent the microvascular endothelial injuries caused by diabetic cardiomyopathy(DCM).In this study,we aimed to investigate the effects of PSS-NP on the dysfunction of cardiac microvascular endothelia in streptozotocin(STZ)-induced DCM rat model.Echocardiographic measurements showed a significant improvement of cardiac function in the PSS-NP-treated group.Our results revealed that the abnormalities of cardiac systolic and diastolic functions were suppressed by the treatments of prostaglandin E1(PGE1),low molecular weight heparin(LMWH),PSS and PSS-NP.Our comparison analysis indicated that PSS-NP showed the strongest inhibitory effects on microvascular endothelial injuries.Transmission electron microscopy analysis demonstrated that PSS-NP protected the cardiac microvascular endothelium and further improved endothelium dysfunction in DCM rats.ELISA and Western blot assays further showed a high efficiency of improving cardiac microvascular endothelial dysfunction with PSS-NP.Our results demonstrated that PSS-NP increased the protein expression of phosphatidylinositol 3-kinase(PI3K)-p85 and vascular endothelial growth factor(VEGF)-A,and the phosphorylation of protein kinase B(Akt)and endothelial nitric oxide synthase(eNOS),which were involved in the amelioration of cardiac microvascular endothelial dysfunction.These data suggest that PSS-NP may be a novel approach to the treatment the coronary microcirculation disorder diseases such as DCM.

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