作者：ZHENGXiaodong; ZHAOJianmin; XIAOShu; W...墨鱼同工酶分析淀粉凝胶电泳技术眼睛肝脏
摘要：Thirty-nine isozymes in four tissues (mantle muscle, buccal bulb muscle, eye and liver) of Sepia esculenta were screened for enzymatic analysis using starch gel electrophoretic technique. Eighteen enzymes (G3PDH, LDH, MDH, MEP,IDHP, PGDH, GRS, NP, AAT, CK, AK, EST, ALP, ACP, FBP, MPI, GPI and PGM)show strong activities and good convergence in zymogram. They are proved to be suitable genetic markers in Sepia esculenta. Among the tissues used, mantle muscle is the best for electrophoretic analysis of isozymes. Eye and liver are fairly good for some special enzymes, such as LDH, EST, MPI, etc. Twenty-six loci are detected. The proportion of polymorphic loci is 0.115 in the Qingdao sample and 0.153 in the Rizhao sample (P＜0.99). The mean values of the observed and expected heterozygosity per locus of Qingdao sample are 0.016 and 0.017, while those of the Rizhao sample are 0.023 and 0.025 respectively.
注：因版权方要求，不能公开全文，如需全文，请咨询杂志社
《中国海洋大学学报·自然科学版》（CN：37-1414/P）是一本有较高学术价值的大型月刊，自创刊以来，选题新奇而不失报道广度，服务大众而不失理论高度。颇受业界和广大读者的关注和好评。 《中国海洋大学学报·自然科学版》主要刊登理、工、农（水产）、医（药）等涉海相关领域中具有原创性的科技论文。坚持为理论研究、高技术开发、成果转化等专家学者、科技工作者服务，以理论研究和知识创新为主导，兼顾应用于开发等国内、国际的最新研究成果。
杂志详情
