作者：Wen; CHEN; Xiangjun; WANG; Yujie; WANG...sipunculusnuduspolysaccharidesresponsesurfaceprocessoptimization
摘要：[Objectives]This study was conducted to optimize experimental conditions for extraction of polysaccharides from Sipunculus nudus.[Methods]With the Sipunculus nudus from Sanya as the experimental material,S.nudus polysaccharide was extracted by ultrasonic microwave assisted method,during which univariate factors including solid-liquid ratio,time,temperature,ultrasonic power,microwave power were investigated,to select optimal extracting conditions,which were also demonstrated and optimized by variance and response surface analysis.The optimized conditions were adopted to carry out a test,in which the absorbance of S.nudus polysaccharide was measured with an ultraviolet and visible spectrophotometer,followed by the calculation of the extracting amount of polysaccharide,and precision test and recovery test were also carried out to verify the experimental method.[Results]The standard solutions were determined at the maximum absorption wavelength of 486 nm,and the regression equation was A=0.267 7 C+0.001 8,R 2=0.999 8.Solid-liquid ratio,time,temperature,ultrasonic power and microwave power were investigated by univariate analysis and then optimized by response surface method as follows:solid-liquid ratio at 1∶20(g/ml),time of 35 min,temperature at 50℃,ultrasonic power at 420W and microwave power at 300 W,under which the measured extracting amount of S.nudus polysaccharide was 1.37 mg/g.The RSD was 0.985%in precision experiment.In the repeatability test,the extracting amounts of polysaccharide were 1.41,1.43 and 1.38 mg/g for three parallel tests,respectively,with a RSD of 1.140%.The recovery test obtained following results:for group a(added with glucose standard):the recovery of S.nudus polysaccharide was in the range of 100.3%-101.4%,RSD=0.03%;and for group b(not added with glucose standard):the recovery of S.nudus polysaccharide was in the range of 99.7%-100.3%,RSD=0.02%.[Conclusions]The research method is stable and reliable.This study provides data basis and reference for follow-up study on S.nudus.
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