作者：Yuefeng; WANG; Fengfeng; XIE; Yuqing; ...marchantiaconvolutadnaextractionsammethodssrprimerscreening
摘要：[Objectives] This study was conducted to study SSR primers in wild Marchantia convoluta by SAM method. [ M ethods] The M. convoluta genomic DNA w a extracted by improved CTAB method，a d the SSR primers for M. convoluta were selected through digestion，ligation, suppression PCR, pre-amplification, SAM method a d PCR. [R eu lts] The improved CTAB method extracted a high purity of DNA； t e SAM PCR method gave clear bands； and 100 positive clones were sequenced，obtaining 86 sequences. [Conclusions] The SSR molecular markers could be used for aalyzing the genetic diversity of M. convoluta.
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