作者：Lech; RODZIEWICZ; Jolanta; MASLOWIECKA...performanceliquidmassspectrometrythyreostatsurine
摘要：Five thyreostats (TSs) , namely tapazole, thiouracil, methylthiouracil, propylthiouracil, and phenylthiouracil,were determined in bovine urine using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) in positive electrospray ionization mode. Extraction and clean-up were achieved using a ChemElut cartridge with tert-butyl methyl ether, without a derivatization step. Separation was achieved on an Acquity UPLC SS T3 column. The mobile phase was acetonitrile and water containing 0.2% (v/v) formic acid. The mass spectrometer was operated in multiple reaction monitoring mode. Urine samples were spiked with TS solution at levels corresponding to 5, 10, 15, and 20 ug/L. The accuracy (internal standard corrected) ranged from 92% to 107%, with a repeatability precision (relative standard deviation,RSD) less than 15% for all five analytes. The RSDs within-laboratory reproducibility was less than 26%. The decision limits (CCa) and detection capabilities (CCp) were obtained from a calibration curve and were in the ranges of 3.1-6.1ug/L and 4.0-7.4ug/L, respectively. The CCa and CCp values were below the recommended concentration, which was set at 10ug/L .The results show that the described method is suitable for the direct detection of TSs in bovine urine. This method can also be used to determine TSs in porcine urine.
注：因版权方要求，不能公开全文，如需全文，请咨询杂志社
