作者：彭化南; 谢嘉霖; 陈熙; 郑劼; 徐和涛; 刘...1crystalstructurebovineserumalbuminfluorescencequenching
摘要：The title compound,methyl 4-(2,4-dichlorophenyl)-2,7,7-trimethyl-5-oxo-l,4,5,6,7,8-hexahydroquinoline-3-carboxylate (C20H21Cl2NO3,1) was synthesized and the crystal structure was determined by single-crystal X-ray diffraction.It crystallizes in tetragonal system,space group P(4)21c with a =16.076(3),b =16.076(3),c =14.750(2) (A),V=3811.8(14)(A)3,Z=8,R =0.0377 and wR =0.0845.The interactions between compound 1 and bovine serum albumin (BSA) were investigated using fluorescence spectroscopy.The results revealed that compound 1 can effectively quench the intrinsic fluorescence of BSA by static quenching mechanism.The thermodynamic parameters revealed that the action forces between compound 1 and BSA were mainly van der Waals forces and hydrogen bonds.According to F(o)rster's non-radioactive energy transfer theory,the binding distance between compound 1 and BSA had been determined.Furthermore,the synchronous fluorescence showed that compound 1 has few effects on the microenvironment and conformation of BSA in the binding process.
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